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Introduction Micromachining MethodsMicromachining of Silicon Micromachining of Glass Micromachining of Fused Quartz (or Fused Silica) Micromachining of Polymeric ChipsMetal Patterning World-to-Chip Interface Microfluidic FlowLiquid Pumping Methods Microfluidic Flow Control Sample IntroductionElectrokinetic Injection Hydrodynamic Injection Other Sample Injection Methods Sample Pre-concentrationSample Stacking Extraction Porous Membrane Other Pre-concentration Methods SeparationGas Chromatography (GC) Capillary Electrophoresis (CE) Chromatographic Separations Coupled Separations Detection MethodsOptical Detection Methods Electrochemical (EC) Detection Mass Spectrometry (MS) Other Detection Methods Applications to Cellular/Particle AnalysisRetention of Cells and Particles Studies of Cells in a Flow Other Cell Operations Applications to Nucleic Acids AnalysisNucleic Acids Extraction and Purification Nucleic Acids Amplification DNA Hybridization Other Nucleic Acid Applications Applications to Protein AnalysisImmunoassay Protein Separation Enzymatic AssaysAppendix Problem Sets Introduction Micromachining Microfluidic Flow Sample Introduction Sample Preconcentration Separation Detection Cellular Analysis Nucleic Acid Analysis Protein Analysis References Glossary